Healing & Recovery
Thymosin Beta-4
Thymosin beta-4 is a 43-amino-acid intracellular peptide that is the primary G-actin sequestering molecule in eukaryotic cells. By regulating actin polymerization dynamics, it influences cell migration, angiogenesis, and wound healing at the fundamental cytoskeletal level. Research has demonstrated its roles in cardiac repair following myocardial infarction, corneal wound healing, and reduction of inflammatory fibrosis. TB-500 is its most commonly used synthetic fragment.
Quick Facts
Also known asTB4, Tbeta4
CategoryHealing & Recovery
Routessubcutaneous, intravenous, topical
Common vial sizes2mg, 3mg, 5mg, 10mg
Molecular weight4963.44 Da
Chain length43 amino acids
SequenceAc-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser
FDA statusNot approved
Storage2-8°C (refrigerated). Reconstituted: 21 days.
Dosing Protocols
| Goal | Dose | Frequency | Route | Cycle | Notes |
|---|---|---|---|---|---|
| Acute Wound Healing | 1.6 mg | 1x/day | subcutaneous | 6 weeks | 1.6mg daily subcutaneous. 2-6 week cycles. |
| Cardiac Protection (Acute) | 42 mg | single dose | intravenous | 0 weeks | 42mg single IV bolus within 6 hours. Phase 2b showed 43% reduction in infarct size. |
| Chronic Tissue Repair | 6 mg | 2x/week | subcutaneous | 12 weeks on / 4 weeks off | 6mg twice weekly subcutaneous. 8-12 week cycles. |
| Neurological Recovery | 30 mg | 3x over 72 hours | intravenous | 0 weeks | 30mg IV infusion, 3 doses over 72 hours. Clinical setting. |
| General Regeneration | 3.5 mg | 1x/day or every other day | subcutaneous | 12 weeks on / 4 weeks off | 2-5mg daily or every other day. 2-12 week cycles. |
| Pressure Ulcer (Topical) | 1x/day | topical | 8 weeks | 1% gel applied once daily. Clinical trials showed 67% complete healing vs 25% placebo. | |
| Corneal Healing (Topical) | 4x/day | topical | 4 weeks | 0.1% ophthalmic drops 4x daily. 6 of 9 subjects achieved complete healing in compassionate use trial. |
Protocols shown are for informational purposes only. Consult a healthcare provider before starting any peptide protocol.
Track Your Thymosin Beta-4 Protocol
Log doses, manage vial inventory, set injection reminders, and visualize blood concentration levels.
Start Tracking — FreeCommon Questions
How do I reconstitute Thymosin Beta-4?
Add bacteriostatic water to your Thymosin Beta-4 vial (common sizes: 2mg, 3mg, 5mg, 10mg). The amount of BAC water depends on your desired concentration. Use our reconstitution calculator for exact measurements.
What is the typical dose of Thymosin Beta-4?
A common dose for Thymosin Beta-4 is 1.6 mg 1x/day via subcutaneous. 1.6mg daily subcutaneous. 2-6 week cycles.
How should I store Thymosin Beta-4?
Store Thymosin Beta-4 at 2-8°C (refrigerated). Full-length thymosin beta-4 (distinct from the TB-500 fragment). Reconstitute with bacteriostatic water. Store lyophilized powder refrigerated. Once reconstituted, use within 21 days.
Is Thymosin Beta-4 FDA approved?
Thymosin Beta-4 is not FDA approved and is available for research purposes only. It has not been evaluated by the FDA for safety or efficacy in humans.
References
- Thymosin beta-4 effective in treatment of heart attack patients - Cardiac Protection Pilot Study (article)
- Thymosin beta 4 ophthalmic solution for dry eye: a randomized, placebo-controlled Phase II clinical trial (article)
- A randomized placebo-controlled single and multiple dose study of intravenous thymosin beta-4 in healthy volunteers (article)
- Treatment of chronic nonhealing neurotrophic corneal epithelial defects with thymosin beta-4 (pubmed)
- Thymosin treatment of venous ulcers - European Clinical Trial (pubmed)
- Pressure Ulcer Clinical Investigation - Phase 2 (pubmed)
- Thymosin beta 4 and the eye: the journey from bench to bedside (article)
- Development of thymosin beta4 for treatment of patients with ischemic heart disease (article)
- Thymosin beta4 and cardiac repair (article)
- The regenerative peptide thymosin beta-4 accelerates the rate of dermal healing (article)