Cognitive
PE-22-28
PE-22-28 is a synthetic heptapeptide that functions as a selective inhibitor of phosphodiesterase-4 (PDE4), an enzyme that degrades cyclic AMP in neural tissue. By increasing intracellular cAMP levels, it enhances CREB-mediated gene transcription associated with memory consolidation and synaptic plasticity. Preclinical studies have demonstrated improvements in long-term memory formation and cognitive performance.
Quick Facts
Also known asMini-Spadin, Shortened Spadin Analog
CategoryCognitive
Half-life23 hours
Routessubcutaneous, oral
Common vial sizes2mg, 3mg, 5mg, 10mg
Molecular weight773.89 Da
Chain length7 amino acids
SequenceGly-Val-Ser-Trp-Gly-Leu-Arg
FDA statusNot approved
Storage2-8°C (refrigerated). Reconstituted: 21 days.
Dosing Protocols
| Goal | Dose | Frequency | Route | Cycle | Notes |
|---|---|---|---|---|---|
| Conservative Research Protocol | 150 mcg | 1x/day | subcutaneous | 8 weeks on / 4 weeks off | 100-200mcg range. Human equivalent theoretical dose. TREK-1 channel blocker with ~300-500 fold greater affinity than full-length Spadin. |
| Standard Antidepressant Research | 100 mcg | 1x/day | subcutaneous | 8 weeks on / 4 weeks off | 50-100mcg range. Antidepressant-like effects seen at 4 days in preclinical models. IC50 of 0.12 nM for TREK-1. |
Protocols shown are for informational purposes only. Consult a healthcare provider before starting any peptide protocol.
Track Your PE-22-28 Protocol
Log doses, manage vial inventory, set injection reminders, and visualize blood concentration levels.
Start Tracking — FreeCommon Questions
How do I reconstitute PE-22-28?
Add bacteriostatic water to your PE-22-28 vial (common sizes: 2mg, 3mg, 5mg, 10mg). The amount of BAC water depends on your desired concentration. Use our reconstitution calculator for exact measurements.
What is the typical dose of PE-22-28?
A common dose for PE-22-28 is 150 mcg 1x/day via subcutaneous. 100-200mcg range. Human equivalent theoretical dose. TREK-1 channel blocker with ~300-500 fold greater affinity than full-length Spadin.
What is the half-life of PE-22-28?
The half-life of PE-22-28 is approximately 23 hours. This determines how long the peptide remains active in your body and influences dosing frequency.
How should I store PE-22-28?
Store PE-22-28 at 2-8°C (refrigerated). Research nootropic peptide. Intranasal administration is commonly used in research protocols. Store reconstituted solution refrigerated. Once reconstituted, use within 21 days.
Is PE-22-28 FDA approved?
PE-22-28 is not FDA approved and is available for research purposes only. It has not been evaluated by the FDA for safety or efficacy in humans.
References
- Shortened Spadin Analogs Display Better TREK-1 Inhibition, In Vivo Stability and Antidepressant Activity (pubmed)
- Spadin, a sortilin-derived peptide, targeting rodent TREK-1 channels: a new concept in the antidepressant drug design (pubmed)
- Deletion of the background potassium channel TREK-1 results in a depression-resistant phenotype (pubmed)
- Fighting against depression with TREK-1 blockers: Past and future. A focus on spadin (article)
- Spadin Selectively Antagonizes Arachidonic Acid Activation of TREK-1 Channels (article)
- Role of TREK-1 in Health and Disease, Focus on the Central Nervous System (pubmed)
- Blocking Two-Pore Domain Potassium Channel TREK-1 Inhibits A1-Like Reactive Astrocyte Activation Through NF-kB Signaling in Rat Depression Model (article)